Step 9: (Week 10) Purification of Expressed Protein using IMAC, Quantification of Expressed Protein, and Evaluation of Sequencing Results (pET15b/LDH) Using BLAST – BioC4025 Biochemistry Lab Manual
His-tagged recombinant protein purification—His60 Ni Superflow Resin
Ni Sepharose™ Excel, 25 ML Cytiva 17371201, 90 μm avg. part. size | Sigma-Aldrich
Ni-NTA Superflow Cartridges
Ni-NTA Superflow (100 ml), 30430 from Qiagen - Labsave
HisPur™ Ni-NTA Superflow Agarose
His-tagged recombinant protein purification—His60 Ni Superflow Resin
Ni-NTA Fast Start Kit
Ni-NTA Superflow 96 BioRobot Kit | Qiagen
His60 nickel resin beats Ni-NTA resin for his-tag purification
protein purification
TALON® Superflow™ Cytiva 28-9575-02, pack of 50 mL | Sigma-Aldrich